SubtiBank SubtiBank
yxeK [2018-02-13T16:46:53.000Z]
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yxeK [2018-02-13T16:46:53.000Z]

similar to monooxygenase, the yxeK-snaB-yxeM-yxeN-yxeO-sndB-yxeQ operon may be implicated in the utilization of a modified cysteine variant
locus
BSU39520
pI
5.67
mw
49.18 kDa
protein length
441 aa Sequence Blast
gene length
1323 bp Sequence Blast
function
sulphur metabolism
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.3|Conversion of S-(2-succino)cysteine to cysteine]
  • [category|SW 2|Metabolism] → [category|SW 2.7|Detoxification reactions]

    • Gene

    Coordinates
    4,060,818 → 4,062,143

    The protein

    Protein family

  • ntaA/snaA/soxA(dszA) monooxygenase family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|8145952307BC47805A7A9BD39418474358043BFE|CmoJ]

    • Structure

  • [PDB|4NBU] (from Bacillus Sp 42% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]-[gene|EBC2B9107AD50DD8F8174FC0BE4AD90DEE6A7CCA|snaB]-[gene|D96EEDABB73AC66E6AB772431819E7C767DC8B9A|yxeM]-[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]-[gene|F8C800CF71D21A1F3265977C4F0EE191D0FFE36A|yxeO]-[gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB]-[gene|7DDB283173267AA106DC277787AAAB4F4F9975F4|yxeQ]
    description
    [Pubmed|10746760]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16513748], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16513748], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]

    • regulation

  • repressed in the presence of cysteine ([protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]) [Pubmed|16513748]

    • additional information

  • the amount of the mRNA is substantially decreased upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B709 (yxeK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1708 NBRP B. subtilis, Japan]
  • BKE39520 (Δ[gene|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGCGATACTCCTTTTAT, downstream forward: _UP4_TATGCAAAGTAAGGAGGAAC
  • BKK39520 (Δ[gene|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGCGATACTCCTTTTAT, downstream forward: _UP4_TATGCAAAGTAAGGAGGAAC

    • References

  • 10746760,16513748,21815947